abstract：:A model has been developed to study the transport of fluid and macromolecules through human arterial umbilical cord endothelial cell monolayers in vitro. Cells were cultured on fibronectin-coated polycarbonate filters and formed within a few days a tight monolayer, with an electrical resistance of 17 +/- 4 Ohm.cm2. Th...

journal_title：Thrombosis and haemostasis

authors： Langeler EG,van Hinsbergh VW

更新日期：1988-10-31 00:00:00

abstract：:Fourteen patients diagnosed as having thrombotic thrombocytopenia purpura (TTP) were studied. Those who survived have been followed during a 1 to 7 year period. The clinical diagnosis was based on changing neurological findings, thrombocytopenia and evidence of microangiopathic hemolytic anemia. Laboratory tests inclu...

journal_title：Thrombosis and haemostasis

authors： Chediak J,Eldridge J,Bergmann F,Sobel D,Baron J,Maxey B,Telfer MC

更新日期：1988-08-30 00:00:00

abstract：:Microvascular thrombosis is considered an important pathogenetic factor in renal failure associated with obstructive jaundice but the mechanisms leading to fibrin deposition are still unknown. The plasma levels of plasminogen activator inhibitor (PAI) in 29 patients with obstructive jaundice were found significantly i...

journal_title：Thrombosis and haemostasis

authors： Colucci M,Altomare DF,Chetta G,Triggiani R,Cavallo LG,Semeraro N

更新日期：1988-08-30 00:00:00

abstract：:Bleeding time and platelet function tests were performed on 31 patients with progressive chronic renal failure (CRF) due to non-immunological (urological) causes, and compared with 22 healthy controls. Patients were classified as mild (plasma creatinine less than 300 mumol/l), moderate (300-600 mumol/l) or severe rena...

journal_title：Thrombosis and haemostasis

authors： Gordge MP,Faint RW,Rylance PB,Neild GH

更新日期：1988-08-30 00:00:00

abstract：:Experiments were carried out to characterize the specificity of uptake of tPA in rat liver cells. Endocytosis in liver endothelial cells of the native carbohydrate variants of tissue plasminogen activator (tPA), and tPA inactivated by diisopropyl fluorophosphate was found to be competitive, suggesting that the determi...

journal_title：Thrombosis and haemostasis

authors： Smedsrød B,Einarsson M,Pertoft H

更新日期：1988-06-16 00:00:00

abstract：:The acidic mucopolysaccharide extracted from sea cucumber (Stichopus japonicus Selenka) (SJAMP) has been shown to cause platelets to aggregate. Using citrated platelet-rich plasma (PRP), washed platelets and formaldehyde-fixed platelets from humans, we investigated the effects of platelet inhibitors and various plasma...

journal_title：Thrombosis and haemostasis

authors： Li JZ,Lian EC

更新日期：1988-06-16 00:00:00

abstract：:Monoclonal antibodies to human fibrin have been prepared from stable hybridomas, obtained by fusion of a mouse myeloma cell line (NS-1) and spleen cells of Balb/c mice immunized with a suspension of human fibrin. One cell line, DG1, producing a monoclonal antibody of the IgG1, kappa subclass, reacted specifically with...

journal_title：Thrombosis and haemostasis

authors： Gargan PE,Ploplis VA,Scheu JD

更新日期：1988-06-16 00:00:00

abstract：:Tissue factor (thromboplastin or Factor III), a glycoprotein cofactor, is required for Factor VII to express its catalytic activity, thereby initiating the extrinsic as well as intrinsic pathway of blood coagulation. Human brain tissue factor was purified 2,500-fold to 98% homogeneity from 2% Triton X-100 extraction o...

journal_title：Thrombosis and haemostasis

authors： Kang S,Niemetz J

更新日期：1988-06-16 00:00:00

abstract：:Desmopressin acetate administration markedly stimulates release of tissue plasminogen activator (t-PA) from vascular endothelial cells. The mechanism for this effect is unknown. Because infusion of epinephrine has been shown to increase t-PA levels, we examined the role of endogenous catecholamine mediation of t-PA re...

journal_title：Thrombosis and haemostasis

authors： Grant MB,Guay C,Lottenberg R

更新日期：1988-04-08 00:00:00

abstract：:An abnormal fibrinogen was discovered in the plasma of a clinically asymptomatic woman. This fibrinogen variant was analyzed by high resolution two-dimensional gel electrophoresis and its molecular abnormality established consisting in a slight decrease in molecular mass of the B beta-chains. Analysis of fibrin reveal...

journal_title：Thrombosis and haemostasis

authors： Meyer M,Schellenberg I,Vogel G,Bischoff I

更新日期：1988-04-08 00:00:00

abstract：:In eighteen patients with fulminant hepatic failure (FHF), in grade III or IV coma, both protein C antigen and activity were significantly decreased (0.35 +/- 0.03 u/ml and 0.35 +/- 0.03 u/ml respectively). There was a significant correlation between protein C antigen and activity (r = 0.61, p less than 0.01). Protein...

journal_title：Thrombosis and haemostasis

authors： Langley PG,Williams R

更新日期：1988-04-08 00:00:00

abstract：:Thrombospondin, a trimeric glycoprotein contained in the platelet alpha-granules, has been proposed as a marker of in vivo platelet activation. However, it is also synthesised by a range of other cells. The extraplatelet contribution to plasma levels of thrombospondin was therefore estimated by investigating the relat...

journal_title：Thrombosis and haemostasis

authors： Dawes J,Pratt DA,Dewar MS,Preston FE

更新日期：1988-04-08 00:00:00

abstract：:The frequency of heterozygous protein C and protein S deficiency, detected by measuring total plasma antigen, in a group (n = 141) of young unrelated patients (less than 45 years old) with venous thrombotic disease was studied and compared to that of antithrombin III, fibrinogen, and plasminogen deficiencies. Among 91...

journal_title：Thrombosis and haemostasis

authors： Gladson CL,Scharrer I,Hach V,Beck KH,Griffin JH

更新日期：1988-02-25 00:00:00

abstract：:In patients with myeloproliferative disorders (MPD) a group of related diseases of the bone marrow stem cell and recurrent haemorrhagic and/or thrombotic complications, the production of aggregating prostaglandins (PGs) may be normal or slightly reduced, while PGI2 production is normal. However, MPD platelet sensitivi...

journal_title：Thrombosis and haemostasis

authors： Cortelazzo S,Galli M,Castagna D,Viero P,de Gaetano G,Barbui T

更新日期：1988-02-25 00:00:00

abstract：:The walls of rabbit mesenteric arterioles and venules (diameter 20 to 40 microns) were punctured with glass micropipets (tip diameter 6 to 8 microns). Thromboembolic reactions resulting from this standardized, small mechanical vessel wall injury could be quantified in vivo with the use of intravital video-microscopy. ...

journal_title：Thrombosis and haemostasis

authors： oude Egbrink MG,Tangelder GJ,Slaaf DW,Reneman RS

更新日期：1988-02-25 00:00:00

abstract：:Calibration of local standards for ristocetin cofactor activity of von Willebrand's factor (vWF:Rcof) against reference preparations is required to achieve a better standardization of this measurement. We have observed that in calibration assays (multiple line parallel bioassay) for vWF:Rcof, measured aggregometricall...

journal_title：Thrombosis and haemostasis

authors： Rodeghiero F,Castaman G

更新日期：1987-12-18 00:00:00

abstract：:The redistribution of platelet glycoproteins (GP) Ib, IIb and IIIa in response to stimulation with human platelet specific alloantibodies, autoantibodies and a quinidine-dependent antibody was investigated using immunofluorescence and a quantitative radioimmunoassay. The platelet GPs carrying the corresponding epitope...

journal_title：Thrombosis and haemostasis

authors： Santoso S,Kiefel V,Mueller-Eckhardt C

更新日期：1987-10-28 00:00:00

abstract：:Glu-plasminogen binds to platelets; the monocytoid line, U937, and the human fetal fibroblast line, GM1380 bind both plasminogen and its activator, urokinase. This study assesses the interaction of these fibrinolytic proteins with circulating human blood cells. Plasminogen bound minimally to red cells but bound satura...

journal_title：Thrombosis and haemostasis

authors： Miles LA,Plow EF

更新日期：1987-10-28 00:00:00

abstract：:In vitro, concentration-dependent effects of rt-PA on a range of coagulation and fibrinolytic assays in thawed plasma samples were investigated. In absence of a fibrinolytic inhibitor, 2 micrograms rt-PA/ml blood (3.4 micrograms/ml plasma) caused prolongation of clotting time assays and decreases of plasminogen (to 44...

journal_title：Thrombosis and haemostasis

authors： Seifried E,Tanswell P

更新日期：1987-10-28 00:00:00

abstract：:Heparin therapy was monitored with the activated partial thromboplastin time (APTT) and with chromogenic substrate assays (factor Xa and factor IIa inhibition) in 100 plasma samples from 47 patients. Heparin concentrations were classified as being below, within or above a defined therapeutic range (TR; 0.2-0.55 units ...

journal_title：Thrombosis and haemostasis

authors： Fey MF,Lang M,Furlan M,Beck EA

更新日期：1987-10-28 00:00:00

abstract：:In conclusion, we believe that the baboon offers many advantages as an experimental animal to study vascular disease, thrombus formation and dissolution, the effects of mediating variables, and the relative efficacy of therapeutic interventions. Each specific application for testing therapeutic agents may require test...

journal_title：Thrombosis and haemostasis

authors： Hanson SR,Harker LA

更新日期：1987-10-28 00:00:00

abstract：:Studies examining the interaction of platelets with exposed subendothelium in vivo have reported conflicting results. To examine possible explanations for the apparently discrepant findings, we measured the platelet reactivity of subendothelium prepared by a number of methods both in vivo and in vitro. In addition, we...

journal_title：Thrombosis and haemostasis

authors： Buchanan MR,Richardson M,Haas TA,Hirsh J,Madri JA

更新日期：1987-08-04 00:00:00

abstract：:Adhesion of rat blood platelets to native rat tail collagen fibrils was studied in the electron microscope under conditions that preserved collagen-associated proteoglycans (CAPG). The CAPG molecules were aligned in chain-like configurations that encircled the fibrils with a 65 nm period; they appeared to coat the fib...

journal_title：Thrombosis and haemostasis

authors： Behnke O

更新日期：1987-08-04 00:00:00

abstract：:Electronic free platelet counting was more sensitive than turbidimetry to detect collagen-induced platelet activation in human platelet-rich plasma. Purified human C1q exhibited a greater inhibitory effect on collagen-induced platelet aggregation in turbidimetry than free platelet counting. Because the change from sma...

journal_title：Thrombosis and haemostasis

authors： Csako G,Suba EA

更新日期：1987-08-04 00:00:00

abstract：:A ternary equimolar human plasmin B-chain-streptokinase-plasminogen complex was isolated from a mixture of the plasmin B-chain-streptokinase complex and human plasminogen at 0 degrees C and 37 degrees C. A ternary complex which was shown to be species specific, was identified and characterized by ultracentrifugal, acr...

journal_title：Thrombosis and haemostasis

authors： Summaria L,Boreisha I,Barlow GH,Robbins KC

更新日期：1987-08-04 00:00:00

abstract：:We studied a murine monoclonal antibody (211 A6) to von Willebrand factor (vWF) with a view to investigating structure-relationship of plasma vWF. The specificity of this antibody has been substantiated by ELISA tests and indirect immunofluorescence. It reacts with purified vWF, normal plasma but not with plasma or pl...

journal_title：Thrombosis and haemostasis

authors： Jorieux S,de Romeuf C,Samor B,Goudemand M,Mazurier C

更新日期：1987-06-03 00:00:00

abstract：:A new model of thrombotic challenge, well suited for screening agents and which acts primarily against platelet thromboembolism, has been used to test the in vivo anti-platelet effects of four calcium channel blockers (CCB). An i.v. injection of a mixture of collagen plus epinephrine (15 micrograms and 1.8 micrograms/...

journal_title：Thrombosis and haemostasis

authors： Ortega MP,Sunkel C,Priego JG,Statkow PR

更新日期：1987-06-03 00:00:00

abstract：:During the collection of samples for plasma beta-thromboglobulin (beta-TG) determination, it is well established that artificially high values can be observed due to in-vitro release. To estimate the reliability of a single beta-TG measurement, blood samples were collected simultaneously from both arms on two separate...

journal_title：Thrombosis and haemostasis

authors： Scarabin PY,Strain L,Ludlam CA,Jones J,Kohner EM

更新日期：1987-04-07 00:00:00

abstract：:The effects of human activated protein C (APC) and thrombin on plasminogen activator inhibitor (PAI-1) released from cultured human umbilical endothelial cells, grown in serum-free 35S-methionine containing medium, were studied in two ways: measurement of PAI-1 activity with an amidolytic assay, and immunoprecipitatio...

journal_title：Thrombosis and haemostasis

authors： de Fouw NJ,van Hinsbergh VW,de Jong YF,Haverkate F,Bertina RM

更新日期：1987-04-07 00:00:00

abstract：:We examined the effect of a synthetic thrombin inhibitor, MCI-9038, on two experimental animal models of disseminated intravascular coagulation (DIC). In a model that DIC induced by the intravenous infusion of thrombin, MCI-9038 suppressed the decrease of platelet count by about 50% at a dose of 0.2 micrograms/kg/min ...

journal_title：Thrombosis and haemostasis

authors： Hara H,Tamao Y,Kikumoto R,Okamoto S

更新日期：1987-04-07 00:00:00

abstract：:The in vitro vitamin K-dependent carboxylation of peptide- or protein-bound glutamate residues is generally studied in detergent-solubilized microsomes from rat or cow liver. Under the conditions usually employed, the efficiency of the carboxylation reaction is low (less than 1% of the carboxylatable residues is conve...

journal_title：Thrombosis and haemostasis

authors： Soute BA,Ulrich MM,Vermeer C

更新日期：1987-02-03 00:00:00

abstract：:In 7 healthy volunteers, formation of thrombin (represented by fibrinopeptide A (FPA) generation, alpha-granule release (represented by beta-thromboglobulin [beta TG] release) and the generation of thromboxane B2 (TxB2) were measured in vivo in blood emerging from a template bleeding time incision. At the site of plug...

journal_title：Thrombosis and haemostasis

authors： Kyrle PA,Westwick J,Scully MF,Kakkar VV,Lewis GP

更新日期：1987-02-03 00:00:00

abstract：:The primary goal of this study was to develop a method for evaluating fibrin-specific antibodies as thrombus detecting agents. The apparatus and assay conditions were chosen by testing antibody 64C5, which binds to the amino terminus of the fibrin beta chain, for its ability to bind to human blood clots. Using 125I-la...

journal_title：Thrombosis and haemostasis

authors： Liau CS,Haber E,Matsueda GR

更新日期：1987-02-03 00:00:00

abstract：:In two randomized double-blind studies perioperative bleeding complications and thromboembolic events were assessed in 189 patients (pts) undergoing elective visceral surgery after subcutaneous administration of a low molecular weight (LMW) heparin fragment (KABI fragment 2165) or unfractionated (UF) heparin. The firs...

journal_title：Thrombosis and haemostasis

authors： Koller M,Schoch U,Buchmann P,Largiadèr F,von Felten A,Frick PG

更新日期：1986-12-15 00:00:00

abstract：:An assay for the quantification of pentosan polysulphate (PPS) in plasma is described. As PPS has been shown to potentiate thrombin inhibition by the second heparin cofactor (HC II), the principle of this assay was to measure the formation of covalent complexes between HC II and the thrombin generated in plasma after ...

journal_title：Thrombosis and haemostasis

authors： Dol F,Sié P,Dupouy D,Boneu B

更新日期：1986-12-15 00:00:00

abstract：:Post-infusion hepatitis is known to occur very frequently in haemophiliacs after treatment with unheated commercial clotting factor concentrates, obtained from large plasma donation pool. On the contrary, single-donor cryoprecipitate is likely to carry a lower risk of transmitting hepatitis. To evaluate this hypothesi...

journal_title：Thrombosis and haemostasis

authors： Morfini M,Rafanelli D,Longo G,Messori A,Ferrini PR

更新日期：1986-12-15 00:00:00

abstract：:We report on three unrelated individuals with the same uncommon type of dysfibrinogenemia, originating from Bergamo, Essen and Perugia. None of them showed bleeding symptoms while the Bergamo patient and members of her family presented with a thrombotic tendency. The presence of a defective fibrinogen was suggested by...

journal_title：Thrombosis and haemostasis

authors： Reber P,Furlan M,Henschen A,Kaudewitz H,Barbui T,Hilgard P,Nenci GG,Berrettini M,Beck EA

更新日期：1986-12-15 00:00:00

abstract：:A new immunoadsorbent-amidolytic assay (IAA) for the specific differential detection of two-chain urokinase-type plasminogen activator (tcu-PA) and its single-chain precursor (scu-PA) in cell culture supernatants has been developed. The assay combines the selectivity of immunoassays with the specificity of enzyme acti...

journal_title：Thrombosis and haemostasis

authors： Corti A,Nolli ML,Cassani G

更新日期：1986-12-15 00:00:00

abstract：:A previous study of neurosurgical patients demonstrated an imbalance between thrombin and plasmin action following surgery. The present study was designed to determine the effect of intermittent pneumatic calf compression on postoperative enzyme activity. Fibrinopeptide A (FPA) and B beta 1-42 levels, reflecting throm...

journal_title：Thrombosis and haemostasis

authors： Weitz J,Michelsen J,Gold K,Owen J,Carpenter D

更新日期：1986-10-21 00:00:00

abstract：:Most of the knowledge acquired on platelet function and biochemistry has been obtained from platelets prepared from blood anticoagulated with sodium citrate. Using washed platelets from human blood (PNB) to which no anticoagulants were added, we report on responses not observed with platelets prepared from citrate-ant...

journal_title：Thrombosis and haemostasis

authors： Bowry SK,Müller-Berghaus G

更新日期：1986-10-21 00:00:00